Journal: Cancer Medicine

Article Title: Upregulated IQUB promotes cell proliferation and migration via activating Akt/ GSK 3β/β‐catenin signaling pathway in breast cancer

doi: 10.1002/cam4.1568

Figure Lengend Snippet: IQUB is significantly upregulated in human breast cancer tissues and cells. A, IQUB protein expression (the brown staining areas) was increased in breast cancer, which was detected in 110 cases of human breast cancer tissue microarray by immunohistochemistry. B, The expression of IQUB in poor differentiation of breast cancer tissues was higher than that in well differentiation of breast cancer tissues. C, IQUB mRNA expression was upregulated in breast cancer tissues (16/20) than paired normal breast tissues which was analyzed by RT ‐ qPCR ( x ‐axis represent 20 pairs of tissue samples). D, IQUB mRNA and protein expression were detected by RT ‐ qPCR and Western blotting in normal breast cell line MCF ‐10A and breast cancer cell lines MCF ‐7 and MDA ‐ MB ‐231. Values represent the mean ± SD from 3 independent measurements. ** P < .01, *** P < .001

Article Snippet: The human breast cancer tissue microarray (the TMA ID: BC081120a) was purchased from Alenabio company (Xi'an, China) which contains 110 cases of breast cancer tissues and corresponding paracancerous tissues.

Techniques: Expressing, Staining, Microarray, Immunohistochemistry, Quantitative RT-PCR, Western Blot

Journal: Molecular Cancer

Article Title: Survivin 2α: a novel Survivin splice variant expressed in human malignancies

doi: 10.1186/1476-4598-4-11

Figure Lengend Snippet: Survivin 2α expression (relative to normal tissue).

Article Snippet: Seven fresh frozen primary medulloblastoma tumor samples were obtained from the Cooperative Human Tumor Network (CHTN), after approval through the Columbus Children's Hospital IRB.

Techniques: Expressing

Journal: Molecular Cancer

Article Title: Survivin 2α: a novel Survivin splice variant expressed in human malignancies

doi: 10.1186/1476-4598-4-11

Figure Lengend Snippet: Expression of survivin splice variants in medulloblastoma (relative to survivin)

Article Snippet: Seven fresh frozen primary medulloblastoma tumor samples were obtained from the Cooperative Human Tumor Network (CHTN), after approval through the Columbus Children's Hospital IRB.

Techniques: Expressing

Journal: Molecular Cell

Article Title: Phosphorylation of PDHA by AMPK Drives TCA Cycle to Promote Cancer Metastasis

doi: 10.1016/j.molcel.2020.09.018

Figure Lengend Snippet: AMPK-PDHc Axis Is Activated in Advanced Breast Cancer Patients, and Its Activation Predicts Poor Metastasis-free Survival (A) PDHA S293 phosphorylation was determined in AMPKα1 knockdown cells. (B) PDHA S293 phosphorylation was determined in control and AMPKα1 knockdown cells with or without A-769662 (100 μM) treatment. (C) The interaction between PDHA and PDHK1 was determined in control and AMPKα1 knockdown cells. (D) The interaction between endogenous PDHA and PDHK1 was determined in control and AMPKα1 knockdown cells upon A-769662 treatment. (E) Immunostaining of p-AMPK (T172) and p-PDHA (S293) in breast cancer tissues with different stages were shown. (F) Scatterplot of pAMPK expression versus pPDHA expression in breast cancer tissues was shown. (G) Kaplan-Meier plots showed high expression of pAMPK and low expression of pPDHA significantly predicted metastasis-free survival. Scale bars indicate 100 μm. See also Figure S4 and .

Article Snippet: Human breast cancer tissues , Chi Mei Medical Center from 1998 to 2004. IRB: 10210004 , N/A.

Techniques: Activation Assay, Phospho-proteomics, Knockdown, Control, Immunostaining, Expressing

Journal: Molecular Cell

Article Title: Phosphorylation of PDHA by AMPK Drives TCA Cycle to Promote Cancer Metastasis

doi: 10.1016/j.molcel.2020.09.018

Figure Lengend Snippet:

Article Snippet: Human breast cancer tissues , Chi Mei Medical Center from 1998 to 2004. IRB: 10210004 , N/A.

Techniques: Virus, Recombinant, Activity Assay, Colorimetric Assay, Stripping Membranes, Plasmid Preparation, Western Blot, Knock-Out, Software, Phospho-proteomics